HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nilsson, F. N. Articles by McGregor, C. G. Search for Related Content PubMed PubMed Citation Articles by Nilsson, F. N. Articles by McGregor, C. G.

The Journal of Thoracic and Cardiovascular Surgery, Vol 102, 923-929, Copyright © 1991 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association

ARTICLES

Methods of cardiac preservation alter the function of the endothelium in porcine coronary arteries

FN Nilsson, VM Miller, PM Vanhoutte and CG McGregor
Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minn. 55905.

This study was undertaken to determine whether clinical methods for preservation and storage of hearts explanted for transplantation affect the responsiveness of coronary arteries to vasoactive agents. Porcine hearts were perfused with crystalloid or blood cardioplegic solution. Rings of coronary arteries were suspended in organ chambers for measurement of isometric force (1) immediately after perfusion and (2) after 5 hours' storage of the hearts at 4 degrees C in the same cardioplegic solution (n = 6 in each group). The maximal contraction of the smooth muscle to potassium chloride, 40 mmol/L, was reduced significantly after perfusion with crystalloid cardioplegic solution (10.8 +/- 1.2 gm) compared with blood cardioplegic solution (17.3 +/- 0.8 gm) and nonperfused coronary arteries (control group 16.9 +/- 1.8 gm). The sensitivity of the arteries with endothelium to the contractile effects of prostaglandin F2 alpha increased after perfusion with crystalloid cardioplegic solution (ED50, [-log mol/L] 5.8 +/- 0.04) compared with blood cardioplegic solution (5.3 +/- 0.02) and the control group (5.7 +/- 0.03). In addition, relaxations to the calcium ionophore A23187, bradykinin, and the alpha 2-agonist BHT-920, which depend on the presence of endothelial cells, were significantly reduced after perfusion with crystalloid compared with blood cardioplegic solution or the control group. The responsiveness of the endothelium and smooth muscle after 5 hours' cold storage was unaltered in the blood cardioplegia group, whereas storage resulted in functional recovery in the crystalloid cardioplegia group, with the result that all groups were comparable. These data suggest an immediate and reversible change in vascular function with crystalloid cardioplegia, which was not apparent with blood cardioplegia.

This article has been cited by other articles:

				Q. Yang and G.-W. He Effect of Cardioplegic and Organ Preservation Solutions and Their Components on Coronary Endothelium-Derived Relaxing Factors Ann. Thorac. Surg., August 1, 2005; 80(2): 757 - 767. [Abstract] [Full Text] [PDF]

				W. Zou, Q. Yang, A. P. C. Yim, and G.-W. He Impaired endothelium-derived hyperpolarizing factor-mediated relaxation in porcine pulmonary microarteries after cold storage with Euro-Collins and University of Wisconsin solutions J. Thorac. Cardiovasc. Surg., July 1, 2003; 126(1): 208 - 215. [Abstract] [Full Text] [PDF]

				Q. Yang, Y.-C. Liu, W. Zou, A. P. C. Yim, and G.-W. He Protective effect of magnesium on the endothelial function mediated by endothelium-derived hyperpolarizing factor in coronary arteries during cardioplegic arrest in a porcine model J. Thorac. Cardiovasc. Surg., August 1, 2002; 124(2): 361 - 370. [Abstract] [Full Text] [PDF]

				A. Parolari, P. Rubini, A. Cannata, L. Bonati, F. Alamanni, E. Tremoli, and P. Biglioli Endothelial damage during myocardial preservation and storage Ann. Thorac. Surg., February 1, 2002; 73(2): 682 - 690. [Abstract] [Full Text] [PDF]

				L. P Perrault, J.-P. Bidouard, P. Janiak, N. Villeneuve, P. Bruneval, J.-P. Vilaine, and P. M Vanhoutte Impairment of G-protein-mediated signal transduction in the porcine coronary endothelium during rejection after heart transplantation Cardiovasc Res, August 1, 1999; 43(2): 457 - 470. [Abstract] [Full Text] [PDF]

				G.-W. He Potassium-channel opener in cardioplegia may restore coronary endothelial function Ann. Thorac. Surg., October 1, 1998; 66(4): 1318 - 1322. [Abstract] [Full Text] [PDF]

				G.-W. He and C.-Q. Yang Impaired Endothelium-Derived Hyperpolarizing Factor-Mediated Relaxation In Coronary Arteries By Cold Storage With University Of Wisconsin Solution J. Thorac. Cardiovasc. Surg., July 1, 1998; 116(1): 122 - 130. [Abstract] [Full Text]

				C. C. Barbe, F. F. Diemont, and P. M. Bonnet Cardioplegia Preserves Hypoxic Response in Isolated Coronary Arteries but Not in Isolated Hearts Ann. Thorac. Surg., May 1, 1997; 63(5): 1303 - 1308. [Abstract] [Full Text]

				M. Struber, K. A. Ehlers, F. N. Nilsson, V. M. Miller, C. G. A. McGregor, and A. Haverich Effects of Lung Preservation With Euro-Collins and University of Wisconsin Solutions on Endothelium-Dependent Relaxations Ann. Thorac. Surg., May 1, 1997; 63(5): 1428 - 1435. [Abstract] [Full Text]

				H. Sato, Z.-Q. Zhao, J. E. Jordan, J. C. Todd, R. D. Riley, C. S. Taft, J. W. Hammon Jr., P. Li, X.-l. Ma, and J. Vinten-Johansen BASAL NITRIC OXIDE EXPRESSES ENDOGENOUS CARDIOPROTECTION DURING REPERFUSION BY INHIBITION OF NEUTROPHIL-MEDIATED DAMAGE AFTER SURGICAL REVASCULARIZATION J. Thorac. Cardiovasc. Surg., February 1, 1997; 113(2): 399 - 409. [Abstract] [Full Text]

				G.-W. He Hyperkalemia Exposure Impairs EDHF-Mediated Endothelial Function in the Human Coronary Artery Ann. Thorac. Surg., January 1, 1997; 63(1): 84 - 87. [Abstract] [Full Text]

				F. W. Sellke, E. M. Boyle Jr, and E. D. Verrier Endothelial Cell Injury in Cardiovascular Surgery: The Pathophysiology of Vasomotor Dysfunction Ann. Thorac. Surg., October 1, 1996; 62(4): 1222 - 1228. [Abstract] [Full Text]

				E. R. Ferguson, R. D. Spruell, W. V. A. Vicente, C. P. Murrah, and W. L. Holman CORONARY VASCULAR REGULATION DURING POSTCARDIOPLEGIA REPERFUSION J. Thorac. Cardiovasc. Surg., October 1, 1996; 112(4): 1054 - 1063. [Abstract] [Full Text]

				G.-W. He and C.-Q. Yang Hyperkalemia Alters Endothelium-Dependent Relaxation Through Non-Nitric Oxide and Noncyclooxygenase Pathway: A Mechanism for Coronary Dysfunction due to Cardioplegia Ann. Thorac. Surg., May 1, 1996; 61(5): 1394 - 1399. [Abstract] [Full Text]

				G.-W. He and C.-Q. Yang Vascular Tone and Contractility During Exposure to Cardioplegia and Hyperkalemic Solutions Vascular and Endovascular Surgery, July 1, 1995; 29(4): 261 - 272. [Abstract] [PDF]

				K. Nakanishi, Z.-Q. Zhao, J. Vinten-Johansen, D. A. Hudspeth, D. S. McGee, and J. W. Hammon Jr. Blood cardioplegia enhanced with nitric oxide donor SPM-5185 counteracts postischemic endothelial and ventricular dysfunction J. Thorac. Cardiovasc. Surg., June 1, 1995; 109(6): 1146 - 1154. [Abstract] [Full Text]