HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Jun Amano Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Amano, J. Articles by Hiroe, M. Search for Related Content PubMed PubMed Citation Articles by Amano, J. Articles by Hiroe, M.

J Thorac Cardiovasc Surg 1997;113:19-025
© 1997 Mosby, Inc.

CARDIAC AND PULMONARY REPLACEMENT

PROLIFERATION OF SMOOTH MUSCLE CELLS IN ACUTE ALLOGRAFT VASCULAR REJECTION

Received for publication March 28, 1996 Revisions requested May 13, 1996 Revisions received June 6, 1996 Accepted for publication June 11, 1996 Address for reprints: Jun Amano, MD, The Second Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390 Japan.

Abstract

Objectives: To determine whether immune injury during acute cardiac rejection induces phenotypic modulation of arterial smooth muscle cells and lesion formation, we studied the expression of embryonic myosin heavy-chain isoform and degrees of intimal proliferation in aortas and coronary arteries of allografted rabbit hearts. Modulation of phenotype in arterial smooth muscle cells during acute vascular injury is a widely reported phenomenon, and proliferation and migration of medial smooth muscle cells contribute to development of intimal hyperplasia of arteries in response to immune injury. Methods: Rabbit hearts were heterotopically transplanted to the neck without immunosuppression. Hearts were harvested at 2, 5, 7, and 10 days after transplantation. Proliferation of smooth muscle cells was assessed by bromodeoxyuridine labeling. Staining for immunohistochemical indicators was done with use of monoclonal antibodies that recognize T lymphocytes and all types of smooth muscle cells (SM1), adult type of smooth muscle cells (SM2), and embryonic myosin heavy-chain isoform. Intimal thickening and luminal narrowing were assessed with a computer-assisted video image analysis system. Results: Intimal thickening and luminal narrowing in aortas and coronary arteries gradually increased in a time-dependent manner. The neointima thus formed consisted of proliferating smooth muscle cells positive for both SM1 and embryonic myosin heavy-chain isoform and massive T lymphocyte accumulation. Intimal proliferation was more prominent in aortas and large epicardial coronary arteries than in the intramyocardial small coronary arteries. Conclusions: These findings suggest that allogeneic immune injury facilitates phenotypic modulation of smooth muscle cells, which may contribute to subsequent transplantation-associated atherosclerosis.

This article has been cited by other articles:

				L. Tang, X. Chen, S. Tang, T. LaLonde, and J. M Gardin Granulation encapsulated stent: a new therapeutic approach for vascular implantation Heart, February 1, 2007; 93(2): 238 - 243. [Abstract] [Full Text] [PDF]

				Y. Zheng, W. T. Weber, S. Wang, A. J. Wein, S. A. Zderic, S. Chacko, and M. E. DiSanto Generation of a cell line with smooth muscle phenotype from hypertrophied urinary bladder Am J Physiol Cell Physiol, July 1, 2002; 283(1): C373 - C382. [Abstract] [Full Text] [PDF]

				S. Sai, A. Iwata, R. Thomas, and M. D. Allen Vascular cell adhesion molecule-1 up-regulation and phenotypic modulation of vascular smooth muscle cells predate mononuclear infiltration in transplant arteriopathy J. Thorac. Cardiovasc. Surg., September 1, 2001; 122(3): 508 - 517. [Abstract] [Full Text] [PDF]

				T. M. Seasholtz, M. Majumdar, D. D. Kaplan, and J. H. Brown Rho and Rho Kinase Mediate Thrombin-Stimulated Vascular Smooth Muscle Cell DNA Synthesis and Migration Circ. Res., May 28, 1999; 84(10): 1186 - 1193. [Abstract] [Full Text] [PDF]

				V. Sauzeau, H. Le Jeune, C. Cario-Toumaniantz, A. Smolenski, S. M. Lohmann, J. Bertoglio, P. Chardin, P. Pacaud, and G. Loirand Cyclic GMP-dependent Protein Kinase Signaling Pathway Inhibits RhoA-induced Ca2+ Sensitization of Contraction in Vascular Smooth Muscle J. Biol. Chem., July 7, 2000; 275(28): 21722 - 21729. [Abstract] [Full Text] [PDF]