HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Jun Nakajima Joel D. Cooper Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakajima, J. Articles by Sundaresan, R. S. Search for Related Content PubMed PubMed Citation Articles by Nakajima, J. Articles by Sundaresan, R. S.

J Thorac Cardiovasc Surg 1999;117:565-571
© 1999 Mosby, Inc.

CARDIOTHORACIC TRANSPLANTATION

Cytotoxic T Lymphocytes Directed Against Donor HLA Class I Antigens On Airway Epithelial Cells Are Present In Bronchoalveolar Lavage Fluid From Lung Transplant Recipients During Acute Rejection

From the Division of Cardiothoracic Surgery,^a,c Department of Surgery and Pathology,^b and the Pulmonary and Critical Care Division^d/Department of Medicine, Washington University School of Medicine, Barnes Hospital, St Louis, Mo.

This work was supported by National Institutes of Health grant HL56643 (T.M.).

Received for publication Aug 3, 1998. Revisions requested Sept 17, 1998. Revisions received Oct 12, 1998. Accepted for publication Oct 12, 1998. Address for reprints: Sudhir Sundaresan, MD, Northwestern University Medical School, 251 East Chicago Ave, Suite 1030, Chicago, IL 60611.

Background: The lung epithelium is among the first donor tissues encountered by the lung allograft recipient's immune system. The purpose of this study was to determine whether lung epithelium was recognized by T lymphocytes that are isolated from bronchoalveolar lavage fluid of lung allograft recipients during periods of acute rejection.
Methods: Lymphocytes isolated from 45 bronchoalveolar lavage samples (from 41 lung transplant recipients) served as effector cells in standard cell-mediated cytolytic assays with several cell lines as targets: BEAS-2B (an immortalized airway epithelial cell line); B-lymphoblastoid cell lines; and K562 (a natural killer–sensitive cell line). Cytotoxic T-lymphocyte activity of bronchoalveolar lavage lymphocytes was correlated with pathologic status.
Results: During acute rejection alone (ie, without concomitant cytomegalovirus infection), mean lysis of the airway epithelial target was significantly greater, compared with during no rejection, when these targets expressed donor-specific HLA class I antigens (P = .007). Lysis of donor class I–matched B-lymphoblastoid cell line targets during rejection was not significantly different from lysis during no-rejection periods (P = .18). Mean lysis of K562, a natural killer cell target, did not differ between acute rejection (without concomitant cytomegalovirus infection) and no rejection (P = .30). During cytomegalovirus infection (without concomitant acute rejection), there was no difference in mean lysis of airway epithelial cells, B-lymphoblastoid cell lines, or K562 targets compared with during no cytomegalovirus infection, whereas during acute rejection, compared with cytomegalovirus infection without rejection, there was a significant increase in mean lysis of the airway epithelial target when it expressed donor-specific HLA antigens (P = .01).
Conclusions: Donor HLA class I–specific cytotoxic T-lymphocyte activity directed at airway epithelial cells was demonstrated in bronchoalveolar lavage lymphocytes from lung transplant recipients. Lysis of these targets was significantly higher during episodes of acute rejection.

This article has been cited by other articles:

				B. Saatian, X.-Y. Yu, A. P. Lane, T. Doyle, V. Casolaro, and E. Wm. Spannhake Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells during differentiation and activation Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L217 - L225. [Abstract] [Full Text] [PDF]

				W. Y. Szeto, A. M. Krasinskas, D. Kreisel, S. H. Popma, and B. R. Rosengard Donor antigen-presenting cells are important in the development of obliterative airway disease J. Thorac. Cardiovasc. Surg., December 1, 2000; 120(6): 1070 - 1077. [Abstract] [Full Text] [PDF]