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J Thorac Cardiovasc Surg 2000;119:921-930
© 2000 The American Association for Thoracic Surgery

General Thoracic Surgery

Comparison of University of Wisconsin, Euro-Collins, low-potassium dextran, And Krebs-Henseleit solutions for hypothermic lung preservation

From the Jewish Hospital Cardiothoracic Surgical Research Institute, Division of Thoracic and Cardiovascular Surgery, Department of Surgery,^a and Department of Anatomical Sciences and Neurobiology,^b University of Louisville, Louisville, Ky.

Supported in part by National Institutes of Health grant GM43890 and a grant from Jewish Hospital Foundation.

Address for reprints: Sufan Chien, MD, Department of Surgery, University of Louisville, School of Medicine, Louisville, KY 40292.

Objective: We sought to test the effectiveness of 4 different solutions for hypothermic rat lung preservation.
Methods: One hundred ninety-two rats were used. The rats were divided into 4 groups, and University of Wisconsin, Euro-Collins, low-potassium dextran, or Krebs-Henseleit solution was used in each group. They were further divided into 6 subgroups of 8 rats each. The lungs were preserved at 4°C for 0, 4, 6, 8, 12, or 24 hours, respectively, and lung function was studied by using a living rat perfusion model.
Results: Pulmonary arterial flow decreased in each group after 4 to 6 hours of preservation; the low-potassium dextran group decreased the least and the Krebs-Henseleit group decreased the most. Pulmonary vascular resistance increased in each group after 6 hours of preservation; the Krebs-Henseleit group increased the most. Although airway pressure increased, static lung compliance and gas exchange capacity decreased after 8 hours of preservation; the Krebs-Henseleit group exhibited the worst values. Lung tissue wet/dry weight ratio increased gradually during preservation; the University of Wisconsin group exhibited the least increase. An ultrastructural study indicated the least morphologic changes in the low-potassium dextran group at 24 hours.
Conclusions: At 4°C, all solutions preserved rat lungs for 4 hours with acceptable function. However, 6 hours of preservation resulted in damaged pulmonary function in some lungs, and this damage increased when preservation time was extended. The lungs preserved in low-potassium dextran solution had the best overall function, but the lungs preserved in University of Wisconsin solution had less edema.

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