HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Otani, H. Articles by Das, D. K. Search for Related Content PubMed PubMed Citation Articles by Otani, H. Articles by Das, D. K.

The Journal of Thoracic and Cardiovascular Surgery, Vol 91, 290-295, Copyright © 1986 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association

ARTICLES

Cardiac performance during reperfusion improved by pretreatment with oxygen free-radical scavengers

H Otani, RM Engelman, JA Rousou, RH Breyer, S Lemeshow and DK Das

We studied the effects of oxygen free radicals on cardiac performance during reperfusion of ischemic myocardium. The pig heart, isolated in situ, was subjected to 60 minutes of regional ischemia at normothermia by occlusion of the left anterior descending coronary artery followed by 60 minutes of hypothermic cardioplegic arrest and 60 minutes of normothermic reperfusion. The oxygen free-radical scavengers, superoxide dismutase and catalase, were administered before occlusion of the left anterior descending coronary artery in the experimental group. The generation of free radicals in the untreated group, estimated by the measurement of malondialdehyde in the perfusate, was significant during reperfusion and was associated with a corresponding increase in creatine kinase. Superoxide dismutase and catalase significantly slowed the appearance of malondialdehyde and the release of creatine kinase during reperfusion. Superoxide dismutase and catalase did not alter coronary flow and myocardial oxygen extraction or consumption during occlusion of the left anterior descending coronary artery; however, coronary flow and oxygen consumption were significantly higher (p less than 0.05) during reperfusion in hearts treated with antioxidants. Left ventricular developed pressure and its maximum first derivative were measured under isovolumic conditions. In the untreated group, left ventricular developed pressure and its maximum first derivative declined to 61.1% and 57.1% of baseline values, respectively, after 60 minutes' occlusion of the left anterior descending, and to 45% of baseline values after 15 minutes of reperfusion. The decline in left ventricular developed pressure and its maximum first derivative during reperfusion was significantly (p less than 0.05) inhibited by superoxide dismutase and catalase, but left ventricular end-diastolic pressure was not significantly altered. These results implicate oxygen-derived free radicals in the injury resulting from reperfusion of ischemic myocardium and suggest that oxygen free- radical scavengers effectively protect against such injury.

This article has been cited by other articles:

				M Larsen, G Webb, S Kennington, N Kelleher, J Sheppard, J Kuo, and J Unsworth-White Mannitol in cardioplegia as an oxygen free radical scavenger measured by malondialdehyde Perfusion, January 1, 2002; 17(1): 51 - 55. [Abstract] [PDF]

				W. Droge Free Radicals in the Physiological Control of Cell Function Physiol Rev, January 1, 2002; 82(1): 47 - 95. [Abstract] [Full Text] [PDF]

				D. K. Das, R. M. Engelman, N. Maulik, J. A. Rousou, J. E. Flack III, and D. W. Deaton Molecular targets of gene therapy Ann. Thorac. Surg., November 1, 1999; 68(5): 1929 - 1933. [Abstract] [Full Text] [PDF]

				K. S. Bolling, A. Halldorsson, B. S. Allen, S. Rahman, T. Wang, M. Kronon, and H. Feinberg PREVENTION OF THE HYPOXIC REOXYGENATION INJURY WITH THE USE OF A LEUKOCYTE-DEPLETING FILTER J. Thorac. Cardiovasc. Surg., June 1, 1997; 113(6): 1081 - 1090. [Abstract] [Full Text]

				J. Iyengar, A. George, J. C. Russell, and D. K. Das Generation of Free Radicals During Cold Injury and Rewarming Vascular and Endovascular Surgery, September 1, 1990; 24(7): 467 - 474. [Abstract] [PDF]